NickelMaghrebV1, Main, Exploration, bibRecord, 000088

Transcriptomic responses to heat stress and nickel in the mussel Mytilus galloprovincialis

Identifieur interne : 000088 ( Main/Exploration ); précédent : 000087; suivant : 000089

Transcriptomic responses to heat stress and nickel in the mussel Mytilus galloprovincialis

Auteurs : Banni Mohamed [Italie, Tunisie] ; Attig Hajer [Tunisie] ; Sforzini Susanna [Italie] ; Oliveri Caterina [Italie] ; Mignone Flavio [Italie] ; Boussetta Hamadi [Tunisie] ; Viarengo Aldo [Italie]

Source :

Aquatic toxicology [ 0166-445X ] ; 2014.

RBID : Pascal:14-0078439

Descripteurs français

Pascal (Inist)
- Choc thermique, Nickel, Moule (comestible), Toxicité, Mytilus galloprovincialis, Effet température, Expression génique, Facteur milieu, Polluant, Métal lourd, Milieu marin, RNA messager, Mollusque comestible, Transcriptomique.
Wicri :
- topic : Nickel, Polluant, Métal lourd, Milieu marin.

English descriptors

KwdEn :
- Edible mollusc, Environmental factor, Gene expression, Heavy metal, Marine environment, Messenger RNA, Mussel, Mytilus galloprovincialis, Nickel, Pollutant, Temperature effect, Thermal shock, Toxicity, Transcriptomics.

Abstract

The exposure of marine organisms to stressing agents may affect the level and pattern of gene expression. Although many studies have examined the ecological effects of heat stress on mussels, little is known about the physiological mechanisms that maybe affected by co-exposure to heat stress and environmental contaminants such as nickel (Ni). In the present work, we investigated the effects of simultaneous changes in temperature and Ni supply on lysosomal membrane stability (LMS) and malondialdehyde accumulation (MDA) in the digestive gland (DG) of the blue mussel Mytilus galloprovincialis (Lam.). To elucidate how the molecular response to environmental stressors is modulated, we employed a cDNA microarray with 1673 sequences to measure relative transcript abundances in the DG of mussels exposed to Ni along with a temperature increase. A two-way ANOVA revealed that temperature and Ni rendered additive effects on LMS and MDA accumulation, increasing the toxic effects of metal cations. Ni loads in the DG were also affected by co-exposure to 26 °C. In animals exposed only to heat stress, functional genomics analysis of the microarray data (171differentially expressed genes (DEGs)) highlighted seven biological processes, largely dominated by the up-regulation of folding protein-related genes and the down-regulation of genes involved in cell migration and cellular component assembly. Exposure to Ni at 18 °C and 26 °C yielded 188 and 262 DEGs, respectively, exhibiting distinct patterns in terms of biological processes. In particular, the response of mussels exposed to Ni at 26 °C was characterized by the up-regulation of proteolysis, ribosome biogenesis, response to unfolded proteins, and catabolic-related genes, as well as the down-regulation of genes encoding cellular metabolic processes. Our data provide new insights into the transcriptomic response in mussels experiencing temperature increases and Ni exposure; these data should be carefully considered in view of the biological effects of heat stress, particularly in polluted areas.

Affiliations:

Italie, Tunisie

Links toward previous steps (curation, corpus...)

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to stream PascalFrancis, to step Checkpoint: 000002
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to stream Main, to step Curation: 000088

Le document en format XML

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<term>Mytilus galloprovincialis</term>
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<front><div type="abstract" xml:lang="en">The exposure of marine organisms to stressing agents may affect the level and pattern of gene expression. Although many studies have examined the ecological effects of heat stress on mussels, little is known about the physiological mechanisms that maybe affected by co-exposure to heat stress and environmental contaminants such as nickel (Ni). In the present work, we investigated the effects of simultaneous changes in temperature and Ni supply on lysosomal membrane stability (LMS) and malondialdehyde accumulation (MDA) in the digestive gland (DG) of the blue mussel Mytilus galloprovincialis (Lam.). To elucidate how the molecular response to environmental stressors is modulated, we employed a cDNA microarray with 1673 sequences to measure relative transcript abundances in the DG of mussels exposed to Ni along with a temperature increase. A two-way ANOVA revealed that temperature and Ni rendered additive effects on LMS and MDA accumulation, increasing the toxic effects of metal cations. Ni loads in the DG were also affected by co-exposure to 26 °C. In animals exposed only to heat stress, functional genomics analysis of the microarray data (171differentially expressed genes (DEGs)) highlighted seven biological processes, largely dominated by the up-regulation of folding protein-related genes and the down-regulation of genes involved in cell migration and cellular component assembly. Exposure to Ni at 18 °C and 26 °C yielded 188 and 262 DEGs, respectively, exhibiting distinct patterns in terms of biological processes. In particular, the response of mussels exposed to Ni at 26 °C was characterized by the up-regulation of proteolysis, ribosome biogenesis, response to unfolded proteins, and catabolic-related genes, as well as the down-regulation of genes encoding cellular metabolic processes. Our data provide new insights into the transcriptomic response in mussels experiencing temperature increases and Ni exposure; these data should be carefully considered in view of the biological effects of heat stress, particularly in polluted areas.</div>
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	Serveur d'exploration sur le nickel au Maghreb
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Serveur d'exploration sur le nickel au Maghreb

Transcriptomic responses to heat stress and nickel in the mussel Mytilus galloprovincialis

Transcriptomic responses to heat stress and nickel in the mussel Mytilus galloprovincialis

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri